| Addition | Conc. (mM) | Relative activity (%) |
| No addition | - | 100 |
| Na+ (NaCl) | 50.0 | 119 |
| K+ (KCl) | 150.0 | 126 |
| Mg2+ (MgCl2) | 40.0 | 70 |
| Ca2+ (CaCl2) | 150.0 | 35 |
| Mn2+ (MnCl2) | 100.0 | 4 |
| Zn2+ (ZnCl2) | 100.0 | 11 |
| Fe3+ (FeCl3) | 100.0 | 5 |
| Ni2+ (NiCl2) | 10.0 | 58 |
| Co (CoCl2) | 10.0 | 34 |
| Cu2+ (CuCl2) | 10.0 | 26 |
| Hg2+ (HgCl2) | 10.0 | 44 |
| 2-mercaptoethanol | 0.5 | 120 |
| SDS | 2.5 | 22 |
| Urea | 2.5 | 58 |
| EDTA | 5.0 | 106 |
| Iodoacetamide | 5.0 | 82 |
| L-cysteine | 25.0 | 104 |
| L-histidine | 25.0 | 113 |
| Glutathione reduced | 0.5 | 115 |
| Substrate | Conc. (mM) | Relative activity (%) |
| L-asparagine | 10 | 100.00 |
| D-asparagine | 10 | 65.00 |
| DL-asparagine | 10 | 77.00 |
| L-glutamine | 10 | N. D. |
| L-aspartic acid | 10 | N. D. |
| D-asparatic acid | 10 | N. D. |
| DL-asparatic acid | 10 | N. D. |
| L-glutamic acid | 10 | N. D. |
| succinamic acid | 10 | 79.00 |
| L-asparagine-t-butyl ester HCL | 10 | 77.00 |
| BOC-L-asparagine | 10 | 88.00 |
| N-alfa-acetyl L-asparagine | 10 | 16.00 |
| L-aspartic acid amide | 10 | 73.00 |